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To perform a serial dilution, a small amount of a well-mixed solution is transferred into a new container and additional water or other solvent is added to dilute the original solution. The diluted sample is then used as the base solution to make an additional dilution. Doing this several times results in a range of concentrations. The initial concentration and target range needed for a given assay determines the size and number of dilution steps required. Often, serial dilutions are performed in steps of 10 or 100.
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Dilution Practice Worksheet
Use a pipette bulb and stay away from the older practice of mouth pipetting. Things Needed. 'How to Make Dilutions.' Sciencing, https.
They are described as ratios of the original and final concentrations. For example, a 1:10 dilution is a mixture of one part of a solution and nine parts of additional solvent. To make a 1:100 dilution, one part of the solution is mixed with 99 parts of additional solvent. Mixing 100 µL of a stock solution with 900 µL of water makes a 1:10 dilution. The final volume of the diluted sample is 1000 µL (1 mL) and the concentration is 1/10 that of the original solution. This is commonly referred to as a 10x dilution.